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Metal Carcinogenesis Testing: Principles and In Vitro Methods (Biological Methods), by Max Costa
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Metal Carcinogenesis Testing explains fundamental principles of metal carcinogenesis as they are currently understood, and provides detailed practical descriptions of rapid and inexpensive in vitro assay methodology presently in use for the detection of potentially carci nogenic metals and their compounds. Mounting experimental evidence has suggested that a number of metals and their compounds are potentially carcinogenic to humans. Since humans are exposed to these potentially carcinogenic metals in industrial situations and through environmental pollution, it is essential that experimental protocols be available to identify the specific metal compounds that are potentially carcinogenic. This book affords a thorough description of the various carcinogenesis test systems available for metals, centering on those that are rapid, inexpensive, and most reliable. The principles are discussed at the level of human exposure, of animal studies, and of research in vitro. Additionally, the molecular mechanisms of metal-induced cancer are considered at each ofthese three levels.In large part, the emphasis rests on the use of in vitro, biochemical and bacterial studies, including tissue culture, because these methods are the basis of the rapid and inexpensive screening of potentially carcinogenic substances.
- Published on: 2013-12-09
- Released on: 2013-12-09
- Original language: English
- Number of items: 1
- Dimensions: 9.02" h x .42" w x 5.98" l, .56 pounds
- Binding: Paperback
- 169 pages
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Page 63 If the metal is attached to a highly lipid soluble moiety, then its dissolution and interaction with biological tissues differs from a metal compound that is relatively lipid insoluble. Page 14 Lipid solubility will allow entrance of the metal by absorption through the skin and blood stream transport. Highly water-soluble metal compounds are not assumed to be very carcinogenic unless they become localized and there is persistent contact with a particular cellular tissue. When eukaryotic cells are broken up, the debris is called Microsomes which contains enzymes such as P450 could be used to explain susceptibility of individual animals to metal carcinogens. Microsomal Benzo[a] pyrene is one of the carcigens found in tobacco smoke to cause cancer. This molecule intercalates in DNA, covalently bonding to the nucleophilic guanine nucleobases and distorts the DNA inducing mutations by perturbing the double-helical DNA structure. This disrupts the normal process of copying DNA and induces mutations, which explains the occurrence of cancer after exposure. This mechanism of action is similar to that of aflatoxin which binds to the N7 position of guanine. See "Cancer Cause and Cure" Yoseph page 65 for further research involving how aflatoxins are involved in cancer. All information is intended for your general knowledge only and is not a substitute for medical advice or treatment for specific medical conditions.
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